diagnosis of human brucellosis by blood culture (bactec) and pcr method via whole blood and serum

Diagnosis of Human Brucellosis by Blood Culture (BACTEC) and PCR Meth- od via Whole Blood and Serum

Background: Brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including Iran. Diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. Rapid detection of Brucella species by an automated blood culture system and Polymerase Chain Reaction (PCR) may ...

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR

direct urease test and acridine orange staining on bactec blood culture for rapid presumptive diagnosis of brucellosis

brucellosis is one of the most common zoonotic diseases in iran and human brucellosis is endemic in all parts of the country. growth of brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. furthermore, in endemic area culture is required for definitive diagnosis. in the present study, direct urease test and acridine orange staining were tried on th...

Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR.

PCR assays for the diagnosis of systemic candidiasis can be performed either on serum or on whole blood, but results obtained with the two kinds of samples have never been formally compared. Thus we designed a nested PCR assay in which five specific inner pairs of primers were used to amplify specific targets on the rRNA genes of Candida albicans, C. tropicalis, C. parapsilosis, C. krusei, and ...

Evaluation of conventional castaneda and lysis centrifugation blood culture techniques for diagnosis of human brucellosis.

We investigated the role of the lysis centrifugation blood culture technique over the conventional Castaneda technique for the diagnosis of human brucellosis. The lysis centrifugation technique has been found to be more sensitive in both acute (20% higher sensitivity; P < 0.00001) and chronic (40% higher sensitivity; P = 0.087) forms of brucellosis. The major advantage of lysis centrifugation w...

Diagnosis of coronavirus by measuring serum concentrations of IL-6 and blood ferritin

Aims:The purpose of this study is to explore changes of pathogenesis markers in peripheral blood of severe COVID-19 patients, which may be of value in disease monitoring. Methods: In this case-control study, we collected data of 180 subjects in two groups including 137 patients with COVID-19 (case) and 43 with non-COVID-19 (control) between March 20 and May 21, 2020, and the clinical characte...